Search results for "chlorophyll fluorescence"
showing 10 items of 105 documents
Measurement and correction of atmospheric effects in O<inf>2</inf>-B and O<inf>2</inf>-A absorption bands in the context of s…
2014
Under sun-light illumination, the shape of the atmospheric oxygen bands (O 2 -B, 687 nm and O 2 -A, 760 nm) of the vegetation radiance is modified by chlorophyll fluorescence. However for far-range measurements, atmospheric effects also modify this shape. In this study, measurements in O 2 -A and O 2 -B absorption bands have been performed at different altitudes up to 3123 m over bare soil and wheat fields. It is observed that bands depth increase significantly with altitude. In O 2 -B band, the total magnitude of variation is of the same order of magnitude as the change induced by vegetation fluorescence, while it is much greater in O 2 -A band. We used MODTRAN 4 to correct measurements fr…
Plant-Based Protein Hydrolysate Improves Salinity Tolerance in Hemp: Agronomical and Physiological Aspects
2021
Hemp (Cannabis sativa L.) is a multipurpose plant attracting increasing interest as a source for the production of natural fibers, paper, bio-building material and food. In this research we studied the agronomical performance of Cannabis sativa cv. Eletta Campana irrigated with saline water. Under those conditions, we tested the effect of protein hydrolysate (PH) biostimulant application in overcoming and/or balancing deleterious salinity effects. The results of the diverse treatments were also investigated at the physiological level, focusing on photosynthesis by means of a chlorophyll a fluorescence technique, which give an insight into the plant primary photochemical reactions. Four sali…
Changes in net photosynthesis, chlorophyll fluorescence and xanthophyll cycle interconversions during freeze-thaw cycles in the Mediterranean moss Le…
1999
The tolerance to freezing and thawing of Leucodon sciuroides, a moss growing in mountainous areas of the Mediterranean (south-east Spain), was investigated by means of CO2 gas exchange, modulated chlorophyll (Chl) a fluorescence and pigment analysis by high-performance liquid chromatography. Evidence is presented for freezing-induced decreases in CO2 fixation that enhance non-radiative dissipation of absorbed light energy, a process which protects the photosynthetic apparatus. The photosynthetic apparatus of L. sciuroides remained fully recuperable after freezing, as indicated by the recovery of photosynthetic CO2 fixation and Chl fluorescence parameters to pre-freezing values during thawin…
Remote sensing of sunlight-induced chlorophyll fluorescence and reflectance of Scots pine in the boreal forest during spring recovery
2005
A measurement campaign to assess the feasibility of remote sensing of sunlight-induced chlorophyll fluorescence (ChlF) from a coniferous canopy was conducted in a boreal forest study site (Finland). A Passive Multi-wavelength Fluorescence Detector (PMFD) sensor, developed in the LURE laboratory, was used to obtain simultaneous measurements of ChlF in the oxygen absorption bands, at 687 and 760 nm, and a reflectance index, the PRI (Physiological Reflectance Index), for a month during spring recovery. When these data were compared with active fluorescence measurements performed on needles they revealed the same trend. During sunny days fluorescence and reflectance signals were found to be str…
Performance of Spectral Fitting Methods for vegetation fluorescence quantification
2010
The Fraunhofer Line Discriminator (FLD) principle has long been considered as the reference method to quantify solar-induced chlorophyll fluorescence (F) from passive remote sensing measurements. Recently, alternative retrieval algorithms based on the spectral fitting of hyperspectral radiance observations, Spectral Fitting Methods (SFMs), have been proposed. The aim of this manuscript is to investigate the performance of such algorithms and to provide relevant information regarding their use. FLD and SFMs were used to estimate F starting from Top Of Canopy (TOC) fluxes at very high spectral resolution (0.12 nm) and sampling interval (0.1 nm), exploiting the O2-B (687.0 nm) and O2-A (760.6 …
Development of the photosynthetic apparatus during light-dependent greening of a mutant of Chlorella fusca.
1977
The formation of chlorophyll, cytochrome f, P-700, ribulose bisphosphate carboxylase as well as photosynthesis and Hill reaction activities were tested during the light-dependent greening process of the Chlorella fusca mutant G 10. Neither chlorophyll nor protochlorophyllide was detected in the darkgrown cells. When transferred to light the mutant cells developed chlorophyll and established its photosynthetic capacity after a short lag phase. In the in vivo absorption spectra a spectral shift of the red absorption peak position from 674 to 680 nm was indicated during the first 3 h of greening. Cytochrome f was already present in the dark-grown cells, but during the greening phase a threefol…
The scattering and re-absorption of red and near-infrared chlorophyll fluorescence in the models Fluspect and SCOPE
2019
Scattering and re-absorption have been recognized as relevant aspects for the interpretation of solar induced chlorophyll fluorescence (SIF) in vegetation remote sensing. In an earlier study [Yang and Van der Tol, RSE 215, 97–108, 2018] we addressed the problem of scattering and re-absorption of near-infrared fluorescence in the vegetation canopy. In this study we analyse within-leaf re-absorption of both red and near-infrared fluorescence using the radiative transfer model Fluspect. The leaf scattering determines the ratio of backward to total leaf fluorescence emission Fb/(Fb + Ff). Fluspect reproduces this ratio with an RMSE of less than 0.1, and explains the observed dependence of the s…
Response of Spinach Leaves (Spinacia oleracea L.) to Ozone Measured by Gas Exchange, Chlorophyll a Fluorescence, Antioxidant Systems, and Lipid Perox…
2004
Spinach (Spinacia oleracea L. cv. Clermont) leaves grown in open-top chambers and exposed to three different concentrations of ozone were measured for gas exchange, chlorophyll a fluorescence, antioxidant systems, and lipid peroxidation at the end of growing season. High O-3 concentration reduced F-v/F-m, indicating that the efficiency in the energy conversion of photosystem 2 (PS2) was altered. The rate of non-cyclic electron transport rate and the capacity to reduce the quinone pool were also affected. The development of non-photochemical quenching was not high enough to decrease the photon excess in the PS2. The limitation of photosynthetic activity was probably correlated with stomata c…
Fluorescence induction kinetics as a tool to detect a chlororespiratory activity in the prasinophycean alga, Mantoniella squamata
1990
Abstract Pulse-modulated fluorescence measuring systems were used to study the fluorescence induction kinetics of the primitive prasinophycean alga, Mantoniella squamata . DCMU-treated cells show a very strong non-photochemical quenching which is insensitive to uncouplers and can not be attributed to state transitions or photoinhibition. In order to analyze the origin for this quenching, different inhibitors of the photosynthetic electron flow were applied. It was found that DCCD, an inhibitor of the ATP-synthase as well as of the cytochrome- c oxidase, enhances the fluorescence quenching, whereas antimycin A, which blocks the cyclic electron flow around PS I, stimulates it. The effect of b…
Linking chlorophyll a fluorescence to photosynthesis for remote sensing applications: mechanisms and challenges
2014
Chlorophyll a fluorescence (ChlF) has been used for decades to study the organization, functioning, and physiology of photosynthesis at the leaf and subcellular levels. ChlF is now measurable from remote sensing platforms. This provides a new optical means to track photosynthesis and gross primary productivity of terrestrial ecosystems. Importantly, the spatiotemporal and methodological context of the new applications is dramatically different compared with most of the available ChlF literature, which raises a number of important considerations. Although we have a good mechanistic understanding of the processes that control the ChlF signal over the short term, the seasonal link between ChlF…